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Vitamin B5 test kit | VitaFast® Pantothenic Acid Vitamin B5 test kit | VitaFast® Pantothenic Acid Vitamin B5 test kit | VitaFast® Pantothenic Acid
Manufacturer: R-Biopharm
Origin: Germany
Art No.: P1005
Test format: Microtiter plate photometer 610-630 nm (alternatively 540-550 nm)
External Certificate AOAC-RI 100904.
P1005 THỰC PHẨM 1 Quantity: 0 cái

Vitamin B5 test kit | VitaFast® Pantothenic Acid

  • Vitamin B5 test kit | VitaFast® Pantothenic Acid
    Manufacturer: R-Biopharm
    Origin: Germany
    Art No.: P1005
    Test format: Microtiter plate photometer 610-630 nm (alternatively 540-550 nm)
    External Certificate AOAC-RI 100904.

1. Intended use

The VitaFast® Pantothenic Acid microtiter plate test is a microbiological method for the quantitative determination of total pantothenic acid (added and natural pantothenic acid) in food, animal feed and in pharmaceutical products. The microbiological test system is in accordance with the international norms.

2. General information

- Pantothenic acid is extracted from a homogenized sample and the extract is diluted. The diluted extract and standards are applied to the wells of a microtiter plate coated with Lactobacillus plantarum. The growth of the bacteria is dependent on the vitamin content of the sample or standard. 

Lactobacillus plantarum belongs to the lactic acid bacteria, and has an absolute growth requirement for the pantothenic acid. Thus, this organism is ideal for quantification of pantothenic acid in food and dietary supplements. As a group, the lactic acid bacteria are good candidates for vitamin analysis due to their genetic stability (resistance to undergo mutation), simple metabolic requirements, ease of maintenance and general safety as a non-pathogenic organism.

3. Benefits

External Certificate AOAC-RI 100904.

- The test system is very robust and well suited for routine analysis.

The VitaFast® test kits feature uniform procedures across the product line.

Accessories

VitaFast® Pantothenic Acid Spiking Standard

4. Specifications

Art. No P1005
Test format  Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation - Liquid samples (added pantothenic acid): sterile filtration and dilution.
- Solid samples (added pantothenic acid): sample homogenization, extraction, centrifugation and dilution.
- Liquid and solid samples (added and native pantothenic acid): sample homogenization, enzymatic treatment, extraction, centrifugation and dilution.
- Incubation time: 20 - 24 h in the dark at 37 °C (98.6 °F)
Evaluation Microtiter plate photometer 610-630 nm (alternativ 540-550 nm)
 
Instructions German/English
MSDS _VitaFast®_Vitamin_B5_IE.pdf (English) sdsP1005_-
LOD (Detection Limit) & LOQ (Limit of quantification - LOD : 0,0035mg/100g (ml)
- LOQ : 0,04mg/100g (ml)
Validated matrices Multivitamin juice, fitness drink, fruit gums, candies, pills, capsules, vitamin mixes, cereals, baby food, bread, flour, meat, meat products, milk products.
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