Oligo-Primer-Probe

We offer a wide range of customizable oligonucleotides—from unmodified primers to over 300 modifications, diverse backbones, and purification options—so you can design oligos with the exact properties you need.
 

Backbones

We offer classic DNA and RNA based oligos, but also modified backbones that grant different properties to your oligos. LNA® backbones increase the affinity and specificity of the oligos. Indeed, their structure confers a very strong thermal stability towards complementary DNA and RNA template suitable for hybridization assays requiring high specificity and/or reproducibility. Both 2’-O-Me RNA backbone and phosphorothioate linkages increase the affinity and specificity for the target, while boosting the resistance to nucleases. 2’-O-MOE RNA backbones are used to increase resistance to nucleases. We also offer PNA backbones well suited for FISH studies. This artificial DNA/RNA analogue confers a higher specificity and sensitivity to the oligo. PNA are also known to be resistant to enzymatic degradation and stable over a wide range of pH, temperature, and salt concentrations.

Modifications

In addition to backbone chemistries, diverse additional modifications can also be incorporated to confer special characteristics to your oligos. For instance, the ability to cross cell membrane can be obtained by adding cholesterol to the oligo, while increasing the affinity and specificity can be achieved with C-5 methylated pyrimidine deoxy-nucleosides, 2’fluoro RNA or methyl-dC, among others. Oligonucleotides can be modified by direct incorporation during the synthesis or by post-synthesis labeling. Direct incorporation Since oligonucleotide synthesis happens from 3’ to 5’, 3’ direct incorporation is not always possible. It depends on the availability of the solid support and compatibility with the chemistries used in the synthesis. 5’ and internal modifications can be introduced using the phosphoramidites, but they need to support harsh cleavagedeprotection conditions. Post-synthesis incorporation Post-synthesis incorporations are used to introduce sensitive dyes or compounds that do not exist as phosphoramidites. It may influence the yield of the reaction. Indeed, a lower yield may result from poly-modifications and/or strong secondary structures. All modifications are available in research, diagnostic and therapeutic grade.

Scales

Purifications

Formats

According to your specifications, oligonucleotides may be provided in various formats. Dried is the default format (except for SePoP unmodified oligonucleotides from 15 to 39 bases). In solution: Select the type of reconstitution buffer (H2 O or TE (pH=8)), determine its volume (ranging from 50 to 1000 μl), and/or specify the final oligonucleotide concentration (ranging from 5 to 250 μM). Annealed: Oligonucleotides are annealed by a short treatment at 94°C followed by a progressive cooling to room temperature. siRNA or cloning linkers are annealed by default. Mixed: Similar amounts of different oligonucleotides can be combined in a single tube. Packaging We provide various packaging options .based on your preferences. 2 mL tube: By default, each oligonucleotide is provided in individual 2 mL tube. Higher volume can be delivered on request (15 mL, 50 mL*). 96-well plates: Cluster tubes, well plates and deep well plates are available for oligos up to 59 bases. Aliquoting: All the oligonucleotides in solution can be split on request in small aliquots of accurate volume (from 50 to 1000 µl). We also offer customized packaging to meet the specific requirements of your project.

qPCR probes

Double-Dye probes

MGB probes

MGB probes are dual-labeled probes incorporating a Minor Groove Binder at their 3´end. MGB Eclipse® probes ordered at Eurogentec are no longer limited by any licence, and can be used for any applications all around the world! With our portfolio of fluorescent dyes for MGB Eclipse® probes, our offer covers all qPCR channels. It will allow you to increase the specificity, sensitivity and efficiency of your assay.

RNAi oligos

RNA Interference (RNAi) is a mechanism of gene silencing at the post transcriptional level, involving RNA molecules such as siRNAs and miRNAs. siRNA Synthetic siRNA duplexes are introduced into cells to cause RNA interference and inhibit the expression of a specific mRNA. Custom siRNA synthesis Typical siRNAs are 21-mer, with a core of 19 double-stranded RNA bases followed by 2 single-stranded DNA nucleotides in 3’, usually Thymidine. Multiple options are available to customize your siRNAs. Reach the level of purity needed by selecting the purification method among SePOP desalting, HPLC (RP or IEX), or our in vivo like purification process. Negative and positive siRNA controls No experiment is complete without proper controls. To monitor your experiment conditions, we provide you with siRNA control duplexes and kits including positive and negative siRNA controls. All siRNA control duplexes are IEX-HPLC purified, and 100 % MALDI-TOF Mass Spectrometry controlled. siRNA controls are annealed and shipped dried. Positive controls: siRNAs targeting a range of endogenous and reporter genes. Each control contains one siRNA duplex. Negative controls: siRNA with no homology with any known eukaryotic gene. The sequence is properly validated. If needed, our experts will help you find the best design for your RNA oligonucleotides.

Eurogentec can support your sequencing projects by offering dedicated NGS grade oligonucleotides.
 

NGS oligos A specific production process to ensure a low cross-contamination (0.1%) for your Next-Generation Sequencing oligos.

Next-Generation Sequencing (NGS) is a high-throughput technology allowing the massive sequencing of nucleic acids following a DNA library preparation. The NGS technology requires that adapter sequences added to fragmented nucleic acids have a high level of purity (no by-products) and ultra-low cross-contamination.
Eurogentec can support your sequencing projects by offering dedicated NGS grade oligonucleotides. Specially produced to avoid cross contamination, they reduce barcode misalignment during multiplex next generation sequencing projects.
 

Aptamers

We collaborate with Novaptech, one of the world leaders in the development of oligonucleotide aptamers. They identify your ideal molecule, we synthesize it. Aptamers are oligonucleotides that bind a specific target molecule, or molecule family, based on their tertiary structure. They are considered as an alternative to antibodies.
Novaptech identifies and optimizes aptamer candidates while Eurogentec is dedicated to synthesizing the final aptamer candidate, along with all oligonucleotides needed for your aptamer development process.
We provide high-value aptamer-related services which include the selection and production of custom optimized RNA and DNA aptamers for analytical, diagnostic, and therapeutic applications. Each aptamer can be provided in various quantities and delivered either unmodified or modified.

Catalog Reagents

We supply oligo synthesis reagents from Glen Research and ready-to-use nucleic acid sequences.

Catalog oligos

In addition to our custom oligonucleotide synthesis service, we also provide ready to use nucleic acid sequences. You can select Universal primers among our catalog. Those pre-designed primers are complementary to nucleotide sequences that occur very commonly in specific sets of DNA molecules and cloning vectors. We also provide Dye-labeled Calibration Oligos used as a reference to calibrate real-time qPCR thermocyclers. In addition, you will find in our catalog PNA-FISH probes for in situ hybridization and negative and positive siRNA controls.