1. Intended use

- The SureFast® Vibrio 4plex (code F5161) is a multiplex real-time PCR for the direct, qualitative detection and differentiation of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus DNA.

- Each reaction contains an internal amplification control (IAC).

2. General information

- The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.

- The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500 and R-Biopharm RIDA®CYCLER.

3. Options

• SureFast® Speed PREP
• SureFast® PREP Bacteria
• SureFast® Mag PREP Pathogens
• TANBead Maelstrom™ 4800

4. Specifications Vibrio PCR kit | SureFast® Vibrio 4plex