Staphylococcus aureus Enterotoxin ELISA Test Kit | RIDASCREEN® SET Total
1. Intended use
RIDASCREEN® SET Total (Code R4105) is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures.
2. General information
- The main causative agent of food poisoning are enterotoxins of Staphylococcus aureus next to the intoxication with Salmonella. Among the strains of Staphylococcus aureus other Staphylococci species as S. hyicus and S. intermedius are able to produce one or more heat stable proteins which behave like enterotoxins.
- Generally, it is assumed that a population of 5 x 105 cells of enterotoxin-producing Staphylococcus aureus strains per gram of food is required to lead to an intoxication. However, other studies showed
- SET intoxications have been frequently associated with pasta, finished meat products, ham, pies, chicken meat products, fish, fish products, milk, milk products, ice-cream, egg products, salads, pastries and cake stuffing as well as preparations from these food products. The enterotoxins of the serological groups A, B, C, D and E are very significant.
3. Benefits
- Official European Screening Method for Staphylococcus enterotoxins.
- Europe-wide ring study according to ISO 16140.
4. Specifications RIDASCREEN® SET Total
Art. No | R4105 |
Test format | Microtiter plate with 96 wells (12 strips with 8 breakable wells each), 96 determinations. |
Sample preparation | "Official European Preparation Method“ (dialysis concentration method) or simplified method. |
Evaluation | Microtiter plate spectrophotometer |
Instructions |
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MSDS | R4105_english.zip (English) |
LOD (Detection Limit) | * Simplified working up: - Liquid samples: 0.25 ng toxin / ml sample, - Solid samples: 0.375 ng toxin / g sample, - Supernatants from bacterial cultures: 0.25 ng toxin / ml sample. * Dialysis concentration: - Liquid samples: 0.05 ng toxin / ml sample, - Solid samples: 0.05 ng t |
Detected analyte | Staphylococcus aureus enterotoxins A, B, C, D, E in total |