Clostridium perfringens PCR kit | SureFast® Clostridium perfringens PLUS
1. Intended use
- The SureFast® Clostridium perfringens PLUS (code F5123) is a real-time PCR for the direct, qualitative detection of a specific alpha-toxin DNA sequence of Clostridium perfringens.
- Each reaction contains an internal amplification control (IAC).
2. General information
- The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time.
- The technical verification of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Applied Biosystems 7500, Bio-Rad CFX96, LTF MyGo, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II, Roche cobas® Z 480 Analyzer and Qiagen Rotor-Gene Q.
3. Accessories
- SureFast® Mag PREP Pathogens
4. Specifications Clostridium perfringens PCR kit | SureFast® Clostridium perfringens PLUS
Art. No | F5123 | |
Test format | 100 reactions/box | |
Sample preparation |
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Instructions | German/English |
MSDS | Statement-MSDS-12-22-NG.pdf (English) |
LOD (Detection Limit) | The SureFast® Clostridium perfringens PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |