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Clostridium botulinum PCR kit | SureFast® Clostridium botulinum Screening PLUS
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Clostridium botulinum PCR kit | SureFast® Clostridium botulinum Screening PLUS
Manufacturer: R-Biopharm
Code: F5110
Test format: 100 reactions/box
1. Intended use
- The SureFast® Clostridium botulinum Screening PLUS (F5110) is a real-time PCR for the direct, qualitative detection of specific Botulinum neurotoxins (BoNT) A, B, E and F DNA sequences of Clostridium botulinum, Clostridium baratii and Clostridium butyricum.
- Each reaction contains an internal amplification control (IAC).
2. General information
- The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time.
- The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q, LTF MyGo Pro and R-Biopharm RIDA®CYCLER.
3. Options
- SureFast® Mag PREP Pathogens
4. Specifications Clostridium botulinum PCR kit | SureFast® Clostridium botulinum Screening PLUS
| Art. No | F5110 |
| Test format | 100 reactions/box |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Bacteria is recommended. |
| Instructions | German/English |
| MSDS | Statement-MSDS-12-22-NG.pdf (English) |
| LOD (Detection Limit) | The SureFast® Clostridium botulinum Screening PLUS real-time PCR has a limit of detection of ≤50 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
