R-Biopharm

SureFast® Escherichia coli PLUS

Intended use:

The test detects Escherichia coli DNA. Each reaction contains an internal amplification control (PLUS).

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions in the FAM and VIC/HEX channel at the same time.
technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche LightCycler® 480 Cobas, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.

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Specifications

Art. No.	F5157
Test format	100 reactions
Sample preparation	For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® PREP Aqua is recommended. To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
Detection limit	The SureFast® Escherichia coli PLUS real-time PCR has a limit of detection of 5 DNA copies.
Cross reactivity	Cross reactivity was observed with DNA extracts from Shigella spp..