SureFood® ALLERGEN Crustaceans

Product detail

Real-time PCR kit for the detection of crustacean DNA in food

Art. No.: S3612

Intended Use

SureFood® ALLERGEN Crustaceans is a real-time PCR kit for the direct, qualitative and/or quantitative detection of specific crustaceans (Crustacea) DNA sequences according to Directive (EC) No. 1169/2011.

The crustacean family contains approximately 44,000 species. Due to the large number of different species, practical verification of all species is not possible. More detailed information is available in the Validation Report.

For quantitative determination, the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301), containing 40 mg crustaceans/kg food sample, and a detection system including a standard series are required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments equipped for simultaneous detection of two fluorescence emission channels, FAM and VIC/HEX.

The instruments technically validated include:

Agilent AriaDx
Bio-Rad CFX96 Dx
Bio-Rad CFX OPUS 96
Qiagen Rotor-Gene Q
R-Biopharm RIDA®CYCLER
Roche LightCycler® 480 II

Specifications

Parameter	Specification
Art. No.	S3612
Test format	100 reactions
Sample preparation	For DNA preparation, the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1, or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Limit of Detection)	≤ 0.4 mg/kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of Quantification)	1 mg/kg using SureFood® PREP Advanced, protocol 1.

Files

Instructions for Use (IFU): German/English.
Material Safety Data Sheet (MSDS/SDS): German and English documents.

For batches placed on the market after January 1, 2024, electronic documentation, including the IFU, SDS, Application Notes and Certificate of Analysis (CoA), is available through the manufacturer's eIFU portal.