SureFast® Listeria monocytogenes PLUS
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Intended use:
SureFast® Listeria monocytogenes PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (PLUS).
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
For users of the Roche LightCyler® 2.0 a color compensation is necessary. The use of the SureCC Color Compensation Kit II is recommended.
Accessories:
Specifications
Art. No. | F5113 |
Test format | 100 reactions |
Sample preparation | For DNA-preparation the use of SureFast® PREP Bacteria is recommended. |
Detection limit | The SureFast® Listeria monocytogenes PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
Files
Instructions |