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SureFast® Listeria monocytogenes PLUS SureFast® Listeria monocytogenes PLUS

Intended use:

SureFast® Listeria monocytogenes PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (PLUS).

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.

For users of the Roche LightCyler® 2.0 a color compensation is necessary. The use of the SureCC Color Compensation Kit II is recommended.

 F5113 LĨNH VỰC KHÁC Số lượng: 0 cái

SureFast® Listeria monocytogenes PLUS

  • Intended use:

    SureFast® Listeria monocytogenes PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (PLUS).

    General information:

    The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.

    For users of the Roche LightCyler® 2.0 a color compensation is necessary. The use of the SureCC Color Compensation Kit II is recommended.

Accessories:

Specifications

Art. No. F5113
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFast® PREP Bacteria is recommended.
Detection limit​ The SureFast® Listeria monocytogenes PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.

Files

Instructions

German/English

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