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Escherichia coli PCR kit | SureFast® STEC 4plex ONE
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Escherichia coli PCR kit | SureFast® STEC 4plex ONE
Manufacturer: R-Biopharm - Germany
Code: F5265
Test format: 100 reactions/box
1. Intended use
The SureFast® STEC 4plex ONE (F5265) can be applied for the fast and simple isolation, detection, and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli serotype O157 in different types of food.
* Two in ONE – STEC 4plex test kit with included DNA preparation
- Integrated DNA preparation
- Use the DNA directly for qPCR
- Open system for all common qPCR thermocyclers
2. General information
- The multiplex real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time.
- The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.
3. Options
4. Specifications Escherichia coli PCR kit | SureFast® STEC 4plex ONE
| Art. No | F5265 |
| Test format | 100 reactions/box |
| Sample preparation | 10 min lysis step |
| Instructions | German/English |
| MSDS | Statement-MSDS-12-22-NG.pdf (English) |
| LOD (Detection Limit) | The SureFast® STEC 4plex ONE real-time PCR has a limit of detection of < 5 DNA copies. |
