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Escherichia coli PCR kit | SureFast® STEC 4plex ONE Escherichia coli PCR kit | SureFast® STEC 4plex ONE Escherichia coli PCR kit | SureFast® STEC 4plex ONE
Manufacturer: R-Biopharm - Germany
Code: F5265
Test format: 100 reactions/box
F5265 THỰC PHẨM 1 Quantity: 0 cái

Escherichia coli PCR kit | SureFast® STEC 4plex ONE

  • Escherichia coli PCR kit | SureFast® STEC 4plex ONE
    Manufacturer: R-Biopharm - Germany
    Code: F5265
    Test format: 100 reactions/box

1. Intended use

The SureFast® STEC 4plex ONE (F5265) can be applied for the fast and simple isolation, detection, and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli serotype O157 in different types of food.

* Two in ONE – STEC 4plex test kit with included DNA preparation

- Integrated DNA preparation

- Use the DNA directly for qPCR

- Open system for all common qPCR thermocyclers

2. General information

- The multiplex real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time.

- The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.

3. Options

RIDA®CYCLER

4. Specifications Escherichia coli PCR kit | SureFast® STEC 4plex ONE

Art. No F5265
Test format 100 reactions/box
Sample preparation 10 min lysis step
 
Instructions German/English
MSDS Statement-MSDS-12-22-NG.pdf (English)
LOD (Detection Limit) The SureFast® STEC 4plex ONE real-time PCR has a limit of detection of < 5 DNA copies.
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