- Trypto Soya Broth 05630 (Soybean Casein Digest Broth) is used for culturing fastidious bacteria such as Neisseria, Brucella, streptococci, and pneumococci.

1. Preparation 

- Add 30 g of the dry medium to 1 L of distilled water, mix well and heat to dissolve.

- Distribute into appropriate containers and sterilise by autoclaving at 121 °C for 15 minutes.

2. Remarks

- The medium is commonly used for culturing fastidious bacteria such as Neisseria, Brucella, streptococci and pneumococci.

- As the bacteria grow rapidly in the medium, they tend to die out quickly, so it is not suitable as a stock culture medium. It is not recommended to subculture pneumococci, streptococci and Neisseria repeatedly on this medium.

- For hemo culture, inoculate 5 ml of blood sample into 50 ml of medium to isolate the bacteria from bacteremia and septicemia.

3. Formula

4. Information Trypto Soya Broth 05630