- TGC Broth is modified for clinical use and capable of growing a wide range of aerobic and anaerobic bacteria.

- It’s also used for blood culture.

1. Preparation

- Add 30 g of the dry medium to 1 L of distilled water, mix well and heat to dissolve. Distribute about 18 mL per tube. Sterilise by autoclaving at 121 °C for 15 minutes, and immediately cool down for use.

- If the medium is stored, re-heat in boiling water to degas, then rapidly cool down before use.

2. Remark

This medium is Vera modifi ed for clinical use and capable of growing a wide range of aerobic and anaerobic bacteria. It’s also used for blood culture. Do not store positive sample in the medium, as they can be destroyed by dextrose fermentation bacteria that grow using dextrose in the medium. It is also possible to culture Trichomonas vaginalis by adding 10% serum to the medium.

3. Formula

4. Information TGC Broth 05629