- GAM Broth (Gifu Anaerobic Medium Broth) contains haemin from digested serum, it is used to cultivate anaerobic bacteria.

- It can also be used to detect other microorganisms such as streptococci, pneumococci and meningococci and is suitable for blood culture.

1. Preparation

- Add 59 g of dry medium to 1L of distilled water and heat to dissolve. Dispense the medium into test tubes or flasks and sterilise by autoclaving at 115 °C for 15 minutes.

- To cultivate anaerobic bacteria, cool down the medium immediately after sterilisation (under running water) without shaking. Inoculate organisms with a sterilised capillary or a Pasteur pipette, and incubate under the anaerobic condition.

2. Remark

As the medium has hemin from digested serum, it is used to cultivate anaerobic bacteria. It could also be used to detect other microorganisms such as streptococci, pneumococci and meningococci and is suitable for blood culture.

3. Formula

4. Informations GAM Broth 05422