Fumonisin Immunoaffinity Column | FUMONIPREP®
1. Intended use
Fumonisin immunoaffinity columns (FUMONIPREP®, code RPRP31) are used in conjunction with HPLC or LC-MS/MS for detection of fumonisins B1 and B2 in a wide range of commodities.
2. General information
- The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins, the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension.
- The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Fumonisins are required to be derivatized when analyzed by HPLC.
High quality:
- Exceeds Official Method criteria
- Manufactured with bespoke automated systems ensuring product consistency also providing unique batch number etching
Value for money:
- Delivering quality at an acceptable cost
- Outstanding technical support from an expert team
- Produced in large batch sizes reducing in-house quality testing
Reliable:
- Consistently high-level performance
- Global mycotoxin test kit supplier for over 35 years
Giving you confidence in the product from a company you can trust!
Accessories
3. Specifications Fumonisin Immunoaffinity Column | FUMONIPREP®
Art. No | RBRP31 / RBRP31B |
Test format | 10 columns (3 ml format) (RBRDP31), 50 columns (3 ml format) (RBRP31B) |
Sample preparation | A representative sample should be obtained by following an officially recognized sampling procedure. The sample is extracted using solvent (Extract solvent can vary depending on the commodity analyzed), filtered, and diluted ready for applying to the immunoaffinity column. |
Evaluation | HPLC or LC-MS/MS. |
Instruction | English |
MSDS |
LOD (Detection Limit) | Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used. HPCL - FB1 and FB2, 3.125 ng/ml LC-MS/MS - FB1 and FB2, 1 ng/ml |
Detected analyte | - Detected analyte: Fumonisin B1, Fumonisin B2 and Fumonisin B3. May also cross-react with other metabolites and masked mycotoxins. |