- Eagle’s MEM2 Powder format 05901 With Kanamycin. Without Phenol Red, L-Glutamine and Sodium Bicarbonate. Autoclavable. This medium promotes the growth of mammalian cells such as HeLa. 

- Eagle's Mem 2 with formulation in powder (100g) permits the preparation of up to 10 L of liquid media.

1. Preparation

- Dissolve 9.4 g of medium in distilled water and adjust the volume to 1 L. Autoclave medium at 121 °C for 15 minutes, then cool down to room temperature. Add sterile 10% sodium bicarbonate solution to get pH 7.1 –7.4 at 37 °C in an atmosphere of 5% CO2.

- This mixture should be capped tightly and stored in a cool and dark place. At the time of use, aseptically add 0.292 g of L-glutamine (sterilised) and the appropriate amount of serum.

2. Summary

- Eagle’s MEM 2 is a powdered medium prepared according to prescription published by Harry Eagle in 1959. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilisation was performed by fi ltration using a membrane filter

- Shimadzu Diagnostics Corporation has succeeded in preparing an autoclavable-powdered medium. This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired. This medium has a growth-promoting effect for cells such as HeLa and other cell lines.

3. Storage and Expiration

Close tightly and store between 2-5 °C in a dark and dry place. May be stored up to one year.

4. Precautions

- Do not use the product after its expiry date. Quality of the product is not warranted after its shelf life. If medium or reagent is in direct contact with eyes or mouth, immediately wash with plenty of water, and consult a physician.

- Any medium, reagent and materials must be sterilised by autoclaving or boiling water after use, and then disposed as industrial waste according to the law and local regulation.

5. Formula

5. Information Eagle's Mem 2 05901