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Eagle’s MEM 2 Powder format 05901 Eagle’s MEM 2 Powder format 05901 Eagle’s MEM2 Powder format 05901
Manufacturer: Shimadzu Diagnostics
Origin: Japan
Code: 05901-EM2-0100
Units: 100g/bottle
05901 Testing laboratories Quantity: 0 cái

Eagle’s MEM 2 Powder format 05901

  • Eagle’s MEM2 Powder format 05901
    Manufacturer: Shimadzu Diagnostics
    Origin: Japan
    Code: 05901-EM2-0100
    Units: 100g/bottle

- Eagle’s MEM2 Powder format 05901 With Kanamycin. Without Phenol Red, L-Glutamine and Sodium Bicarbonate. Autoclavable. This medium promotes the growth of mammalian cells such as HeLa. 

- Eagle's Mem 2 with formulation in powder (100g) permits the preparation of up to 10 L of liquid media.

1. Preparation

Dissolve 9.4 g of medium in distilled water and adjust the volume to 1 L. Autoclave medium at 121 °C for 15 minutes, then cool down to room temperature. Add sterile 10% sodium bicarbonate solution to get pH 7.1 –7.4 at 37 °C in an atmosphere of 5% CO2.

- This mixture should be capped tightly and stored in a cool and dark place. At the time of use, aseptically add 0.292 g of L-glutamine (sterilised) and the appropriate amount of serum.

2. Summary

Eagle’s MEM 2 is a powdered medium prepared according to prescription published by Harry Eagle in 1959. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilisation was performed by fi ltration using a membrane filter

- Shimadzu Diagnostics Corporation has succeeded in preparing an autoclavable-powdered medium. This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired. This medium has a growth-promoting effect for cells such as HeLa and other cell lines.

3. Storage and Expiration

Close tightly and store between 2-5 °C in a dark and dry place. May be stored up to one year.

4. Precautions

Do not use the product after its expiry date. Quality of the product is not warranted after its shelf life. If medium or reagent is in direct contact with eyes or mouth, immediately wash with plenty of water, and consult a physician.

- Any medium, reagent and materials must be sterilised by autoclaving or boiling water after use, and then disposed as industrial waste according to the law and local regulation.

5. Formula

Công thức Eagle's Mem 2 05901

5. Information Eagle's Mem 2 05901

Thông tin Eagle's Mem 2
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