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DON Immunoaffinity Column | DONRHONE® WIDE DON Immunoaffinity Column | DONRHONE® WIDE DON Immunoaffinity Column | DONRHONE® WIDE
Manufacturer: R-Biopharm
Origin: Germany
Code: RBRP141/100
Pack size: 100 column/box
RBRP141/100 Testing laboratories Quantity: 0 cái

DON Immunoaffinity Column | DONRHONE® WIDE

  • DON Immunoaffinity Column | DONRHONE® WIDE
    Manufacturer: R-Biopharm
    Origin: Germany
    Code: RBRP141/100
    Pack size: 100 column/box

1. Intended use

DON immunoaffinity columns (DONRHONE® WIDE, code RBRP141/100) for use in conjunction with an HPLC or LC-MS/MS for detection of deoxynivalenol in a wide range of commodities.

2. General information

The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins, the sample extract is filtered, diluted, and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatized when analyzed by HPLC.

The total extraction and clean-up time takes approximately 20 minutes to perform. The result is improved clean-up and concentration of the toxins from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for large-scale analysis of samples.

High quality:

Exceeds Official Method criteria

Manufactured with bespoke automated systems ensuring product consistency also providing unique batch number etching

Value for money:

Delivering quality at an acceptable cost

Outstanding technical support from an expert team

Produced in large batch sizes reducing in-house quality testing

Reliable:

Consistently high level performance

Global mycotoxin test kit supplier for over 35 years

3. Specifications DON Immunoaffinity Column | DONRHONE® WIDE

Art. No RBRP141/100
Test format 100 columns (3 ml format)
Sample preparation A representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent (Extraction solvent can vary depending on commodity analysed), filtered and diluted ready for applying to the immunoaffinity column.
Evaluation HPLC or LC-MS/MS.
 
Instruction English
MSDS  
LOD (Detection Limit) Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.
DON, 25 ng/ml
Available application notes

- Detected analyte: Deoxynivalenol and 3-acetyl DON. May also cross react with metabolites and masked mycotoxins.
- Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.

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