1. Intended use

DON immunoaffinity columns (DONRHONE® WIDE, code RBRP141/100) for use in conjunction with an HPLC or LC-MS/MS for detection of deoxynivalenol in a wide range of commodities.

2. General information

- The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins, the sample extract is filtered, diluted, and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatized when analyzed by HPLC.

- The total extraction and clean-up time takes approximately 20 minutes to perform. The result is improved clean-up and concentration of the toxins from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for large-scale analysis of samples.

High quality:

- Exceeds Official Method criteria

- Manufactured with bespoke automated systems ensuring product consistency also providing unique batch number etching

Value for money:

- Delivering quality at an acceptable cost

- Outstanding technical support from an expert team

- Produced in large batch sizes reducing in-house quality testing

Reliable:

- Consistently high level performance

- Global mycotoxin test kit supplier for over 35 years

3. Specifications DON Immunoaffinity Column | DONRHONE® WIDE