Intended use:

Easy to use microbiological microtiter plate test for the quantification of total vitamin B6 (enriched and natural vitamin B6) in food, feed and pharmaceutical products.

General information:

Vitamin B6 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B6 assay-medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of vitamin B6. Following the addition of vitamin B6 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B6 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).