Intended use:

Easy to use microbiological microtiter plate test for the quantification of total vitamin B1 (enriched and natural vitamin B1) in food, feed and pharmaceutical products.

General information:

Vitamin B1 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B1-assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus fermentum. The growth of Lactobacillus fermentum is dependent on the supply of vitamin B1. Following the addition of vitamin B1 as a standard or as a compound of the sample, the bacteria grows until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44-48 h. The intensity of metabolism or growth in relation to the extracted vitamin B1 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).