Intended use:

Easy to use microbiological microtiter plate test for the quantification of total inositol (added and native inositol) in food, animal feed and pharmaceutical products.

General information:

Inositol is extracted from the sample and the extract is diluted. The diluted extract and the inositol assay – medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of inositol. Following the addition of inositol as a standard or as a compound of the sample, the bacteria grow until the inositol is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth of Saccharomyces cerevisiae in relation to the extracted inositol is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).