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SureFood® Oat SureFood® Oat

Intended use:

The test detects oat DNA (Avena sativa). Each reaction contains an internal amplification control.

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q and LTF MyGo Pro.

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    SureFood® Oat

    • Intended use:

      The test detects oat DNA (Avena sativa). Each reaction contains an internal amplification control.

      General information:

      The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q and LTF MyGo Pro.

      Specifications

      Art. No. P1010
      Test format 100 reactions
      Sample preparation For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
      Detection limit The SureFood® Oat real-time PCR has a limit of detection of  500 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.

      Files

      Instructions English

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