SureFast® Legionella 3plex
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Intended use:
The SureFast® Legionella 3plex is a real-time PCR for the direct, qualitative detection of Legionella spp. and Legionella pneumophila.
Each reaction contains an internal amplification control.
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC/HEX and Cy5) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Applied Biosystems 7500 and Qiagen RotorGene Q.
Specifications
Art. No. | F5505 |
Test format | 100 reactions |
Sample preparation | For DNA-preparation the use of SureFast® PREP Aqua is recommended. |
Detection limit | The SureFast® Legionella 3plex real-time PCR has a limit of detection of 5 DNA copies. |
Files
Instructions |