R-Biopharm

SureFast® Yersinia enterocolitica PLUS

Intended use:

SureFast® Yersinia enterocolitica PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Yersinia enterocolitica virulence-associated gene ail.

Each reaction contains an internal amplification control (PLUS).

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen RotorGene Q and LTF MyGo Pro.

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Product detail

Specifications

Art. No.	F5124
Test format	100 reactions
Sample preparation	For DNA-preparation the use of SureFast® PREP Bacteria is recommended.
Detection limit	The SureFast® Yersinia enterocolitica PLUS real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.