RIDA®CYCLER 4-channel real-time PCR instrument – R-Biopharm

Overview

RIDA®CYCLER is a multiplex real-time PCR/quantitative PCR (qPCR) instrument using magnetic induction technology for applications with SureFast® and SureFood® kits. It combines a compact design, rapid temperature changes and user-friendly software.

Features and benefits

• Compact, portable design suited to laboratory workspaces.
• Magnetic induction technology combined with fan-forced air cooling enables rapid heating and cooling.
• High-speed centrifugation at the start of a run brings samples and reaction components to the bottom of the tubes, avoiding a separate centrifugation step.
• Tubes are pre-loaded with an oil overlay to prevent evaporation and condensation during the reaction.
• User-friendly software with pre-loaded thermal profiles for SureFast® and SureFood® kits.
• Up to 10 instruments can be connected to one computer via Bluetooth or USB.

Technical specifications

Optical channels

Ex denotes the excitation wavelength; Em denotes the emission wavelength.

Minimum computer requirements

The configuration below follows the supplied catalogue. Confirm the operating system and software version compatible with the configuration in use.

Calibration

According to the catalogue, RIDA®CYCLER requires no calibration thanks to its non-contact induction heating system.

RIDA®CYCLER software

The software allows reports to be configured as needed and exported in PDF format.

The software includes pre-loaded thermal profiles for assays using SureFast® and SureFood® kits. A qPCR run is started in four steps:

Step 1: Select the sample test type.

Step 2: Select the corresponding assay program.

Step 3: Enter the sample names or identifiers.

Step 4: Start the qPCR run.

Result analysis

Cycling analysis

Determine the quantification cycle (Cq)/cycle threshold (Ct) and reaction efficiency for each sample.

Absolute quantification

Determine the unknown amount or concentration of a sample using a standard curve generated from samples with known amounts or concentrations.

Applications with SureFast® and SureFood® kits

The catalogue presents SureFast® and SureFood® real-time PCR kits validated on RIDA®CYCLER for the following application areas:

• Allergen detection.
• Genetically modified organism (GMO) detection.
• Animal species identification.
• Microorganism detection.

Included in delivery

According to the March 2018 brochure, the RIDA®CYCLER instrument, order code ZRCYCLER, is supplied with one box of tubes.

Accessories and consumables

RIDA®CYCLER-MIC-Tubes — order code ZRC-MIC-TUBES: box of 960 tubes and caps. This accessory can be ordered separately, as listed in the March 2018 brochure.