FastGene qFYR Plus Real-Time PCR System

FastGene® qFYR Plus – 6-Channel Real-Time PCR System

96-Well Real-Time PCR System | 6 Fluorescence Channels | Multiplex qPCR

The FastGene® qFYR Plus (FG-QPTC02) from Nippon Genetics Europe is a real-time fluorescent quantitative PCR (qPCR) system designed for the sensitive detection and quantification of nucleic acids.

Featuring a high-sensitivity optical detection system, a 96-well thermal block with gradient functionality, and six fluorescence channels, the qFYR Plus supports a wide range of molecular biology and nucleic acid analysis applications.

1. Key Features

• Fast Detection: Supports probe-based and dye-based qPCR assays.
• 6 Fluorescence Channels: Designed for multiplex qPCR, enabling the detection of up to six different targets in a single reaction well with compatible assay designs.
• Standard 96-Well Format: Features a 96-well thermal block with a temperature gradient function for optimizing primer annealing temperatures.
• High Sensitivity: A high-sensitivity optical detection system enhances fluorescent signal detection.
• Outstanding Temperature Uniformity: Provides consistent amplification conditions across all wells of a 96-well plate.
• Intuitive Software: Includes easy-to-use analysis software with customizable settings and integrated analysis algorithms.
• Integrated Precision Melt Analysis Tool: Supports probe-based allelic discrimination and melting curve analysis.

2. Innovative Technology

High-Sensitivity Optical Detection

The FastGene® qFYR Plus features a unique, patented optical detection system combining a high-quality MPPC detector with a Fresnel lens featuring a short focal length.

The short distance between the detector and the sample helps reduce signal loss and cross-talk between wells, improving fluorescence detection sensitivity.

Precise and Uniform Thermal Cycling

The system uses advanced Peltier technology with six temperature control modules. Its hollowed-out thermal block design reduces overall block weight and enables rapid heating and cooling.

Combined with edge temperature compensation technology, the system provides precise temperature control and uniformity across the 96-well plate, supporting reliable and consistent qPCR results.

Key thermal performance specifications include:

• Maximum block heating and cooling rate: 6.0 °C/s.
• Average sample heating and cooling rate: 4.0 °C/s.
• Temperature accuracy: ±0.2 °C.
• Temperature uniformity: ±0.2 °C.

Six Fluorescence Channels for Multiplex qPCR

The FastGene® qFYR Plus is equipped with six fluorescence channels, enabling the detection of up to six different targets in a single reaction well.

The channels support commonly used fluorescent dyes, including:

• Channel 1: FAM/SYBR Green.
• Channel 2: VIC/JOE/HEX/TET.
• Channel 3: ROX/Texas Red.
• Channel 4: Cy5.
• Channel 5: Cy5.5/Quasar 705/Alexa Fluor 680.
• Channel 6: ATTO 425.

The system uses six high-efficiency monochromatic LEDs as excitation light sources. In standard mode, a full-channel scan takes approximately 8.5 seconds per 96-well plate.

3. FastGene® qFYR Analysis Studio

The FastGene® qFYR Analysis Studio software features an intuitive user interface, clearly structured menus, and customizable settings to accommodate different experimental setups.

Key software features include:

• Automatic data analysis using integrated algorithms.
• Automatic baseline subtraction and Cq threshold calculation.
• Absolute and relative nucleic acid quantification.
• Saved analysis settings for repeated workflows.
• Automatic export of experimental data to Excel, PDF, and TXT formats.
• Integrated Precision Melt Analysis Tool for probe-based allelic discrimination.

4. Applications

The FastGene® qFYR Plus supports a broad range of real-time PCR applications in molecular biology and nucleic acid analysis, including:

• Gene expression analysis.
• Absolute and relative quantification.
• Endpoint qualitative analysis.
• Genotyping.
• Gene mutation analysis.
• Pathogen detection.
• Genetically modified organism (GMO) detection.
• Protein stability screening.
• microRNA (miRNA) analysis.
• Melting curve analysis and high-resolution melting (HRM).

5. Specifications

Thermal Cycler

Optical Detection

Physical Specifications

6. Compatible Consumables

The FastGene® qFYR Plus is compatible with low-profile (0.1 mL) PCR tubes and 8-well PCR tube strips with transparent, flat optical caps, as well as low-profile 96-well PCR plates in non-skirted or semi-skirted formats.

Single PCR Tubes

• Low-profile (0.1 mL) clear or white thin-walled PCR tubes with flat, transparent optical caps.
• Example: PCR Tubes 0.1 mL with flat caps – FG-011F.

8-Well PCR Tube Strips

• 8-well PCR Tube Strips 0.1 mL with flat cap strips – FG-017FC.
• 8-well PCR Tube Strips 0.1 mL with flat caps – FG-018WF.
• 8-well White PCR Tube Strips 0.1 mL + optical Flat Cap Strips – FG-019FC.

96-Well PCR Plates

• FastGene® White 96-well PCR Plate 0.1 mL, semi-skirted – FG-2102.
• FastGene® 96-well PCR Plate 0.1 mL, non-skirted – FG-170350.
• FastGene® 96-well Fast PCR Plate 0.1 mL – FG-03890-50.

Compatibility note: The system is not compatible with high-profile (0.2 mL) PCR tubes or convex tube caps.

7. Product Information

Product name: FastGene® qFYR Plus Real-Time PCR System
Brand: FastGene® – Nippon Genetics Europe
Cat. No.: FG-QPTC02
Configuration: 96-well real-time PCR system with 6 fluorescence channels.

The FastGene® qFYR Plus is a real-time PCR solution for laboratories requiring sensitive nucleic acid quantification, precise temperature control, and multiplex detection capabilities. With six fluorescence channels, an advanced optical detection system, and integrated analysis software, it supports a wide range of qPCR applications on a single platform.

Contact us for technical consultation and a quotation for the FastGene® qFYR Plus (FG-QPTC02).

Pacificlab – Official Distributor of Nippon Genetics Europe in Vietnam

☎️ 0989 092 258
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