Eurogentec
qPCR Probe Design and Synthesis Services
Custom qPCR probe design and synthesis services offering a range of probe types, fluorescent labels, and quenchers to support nucleic acid detection and quantification in molecular biology research and assay development.
Manufacturer: Eurogentec
Origin: Belgium
Product detail
CUSTOM qPCR & dPCR PROBES – EUROGENTEC
1. Product Overview
Eurogentec Custom qPCR & dPCR Probes are custom-synthesized oligonucleotide probes designed according to customer-specific sequences and application requirements for quantitative real-time PCR (qPCR) and digital PCR (dPCR).
With oligonucleotide synthesis expertise dating back to 1985, Eurogentec offers a broad portfolio of dual-labeled probes, Minor Groove Binder (MGB) probes, and Molecular Beacons. Its extensive selection of fluorescent dyes and quenchers allows researchers to select probe configurations compatible with their fluorescence detection systems and assay designs.
Available options include different sequence lengths, fluorescent labels, quenchers, purification methods, and delivery formats to support molecular biology research and molecular assay development.
2. Probe Types
2.1. Double Dye Probes
Double Dye Probes incorporate a fluorescent reporter dye and a quencher at the 5′ and 3′ ends, respectively. This design supports fluorescence-based target detection in probe-based qPCR and dPCR assays.
Eurogentec offers probes with conventional DNA or LNA® (Locked Nucleic Acid) backbones, together with a range of reporter–quencher combinations selected according to the instrument and assay requirements.
Examples include FAM/BHQ®-1, FAM/TAMRA, HEX/EDQ, and ROX/BHQ®-2.
2.2. Minor Groove Binder (MGB) Probes
Minor Groove Binder (MGB) Probes are dual-labeled probes incorporating a Minor Groove Binder moiety at the 3′ end. According to Eurogentec, this design can help improve assay specificity and sensitivity, particularly in applications requiring discrimination between closely related nucleic acid sequences.
Eurogentec offers MGB Eclipse® probes with a selection of fluorescent labels for different qPCR detection channels. The appropriate configuration should be selected according to the target sequence, instrument, and assay requirements.
2.3. Molecular Beacon Probes
Molecular Beacons are hairpin-structured nucleic acid probes. Their fluorescence response changes upon interaction with a complementary target sequence, enabling target detection in suitable molecular biology assays.
Eurogentec offers Standard Molecular Beacons, Wavelength-Shifting Molecular Beacons, and 2′-O-Me RNA Molecular Beacons. Custom-synthesized probes are available with the non-fluorescent quenchers DABCYL or BHQ®, with other dyes available upon request.
3. Key Features
- Multiple Probe Formats: A diverse range of probe types, including Double Dye Probes, MGB Probes, and Molecular Beacons.
- Custom Synthesis: Probes designed according to customer-specific nucleic acid sequences and selected labeling configurations.
- Extensive Dye and Quencher Selection: A wide range of fluorescent dyes and quenchers to support compatibility with different fluorescence detection channels.
- Quality Control: Quality control using MALDI-TOF MS and UHPLC, as specified for the respective probe types.
- Multiple Delivery Options: Available in lyophilized or solution formats, depending on the probe type.
Support Across Development Stages: Oligonucleotides suitable for research, preclinical and diagnostic applications, depending on the product and its intended use.
4. Technical Specifications
Specification | Double Dye Probes | MGB Probes | Molecular Beacons |
|---|---|---|---|
Length | 8–45 bases | 8–30 bases | 25–50 bases |
Purification | HPLC | HPLC | HPLC |
QC | MALDI-TOF MS + UHPLC | MALDI-TOF MS + UHPLC | MALDI-TOF MS + UHPLC |
Backbone | DNA, LNA® | DNA | DNA, LNA® |
Format | Lyophilized in individual tubes or supplied in H₂O/TE solution | Supplied in H₂O/TE solution; dry format or 10 nmol aliquots available upon request | Supplied in H₂O/TE solution or lyophilized in individual tubes |
Quantity | 10, 40, 200, 1.000 nmol (Synthesis scale) | 6, 20, 50 nmol (Synthesis scale) | 40, 200, 1.000 nmol; 2,5, 5, 10 µmol (Synthesis scale) |
6. Selecting the Right Probe
When selecting the appropriate probe configuration, the following factors should be considered:
- Probe Type: Double Dye Probes, MGB Probes, or Molecular Beacons.
- Target Sequence: The nucleic acid sequence to be detected and any relevant design requirements.
- Fluorescent Dye: Compatibility with the detection channels of the qPCR/dPCR instrument.
- Quencher: Selection based on the fluorescent dye and the compatibility of the dye–quencher pair.
- Length and Modifications: Selection according to the assay design requirements and desired assay performance.
- Quantity and Purification: Selection based on the requirements of research, assay optimization, or assay development.
Final selection should be based on assay design, instrument specifications, and the manufacturer's technical recommendations.
7. Contact Us for Technical Support and Quotation
Looking for custom qPCR or dPCR probes for your research or assay development project? Contact us for assistance in selecting the appropriate probe type, fluorescent dye, quencher, purification method, and delivery quantity for your application.
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