Immunoaffinity column for the simultaneous analysis of Biotin, Vitamin B12 and folic acid | EASI-EXTRACT® MULTI-VIT B (LGE)

1. Intended use

- Immunoaffinity column for the simultaneous analysis of Biotin, Vitamin B12 and folic acid, used in combination with an HPLC or LC-MS/MS system to detect biotin, folic acid and vitamin B12 in a wide range of commodities.

2. General information

- The immunoaffinity column is used in combination with an HPLC or LC-MS/MS system to detect biotin, folic acid and vitamin B12 in a wide range of commodities.

- After the vitamins have been extracted, the sample extract is diluted with buffer and filtered before being passed slowly through the immunoaffinity column. Any vitamins present in the sample are retained by the antibodies in the gel suspension. The column is washed to remove any unbound material and the vitamins are then released from the column after elution with solvent. The eluate is collected, evaporated and reconstituted before analysis by HPLC or LC-MS/MS.

* Advantages of the immunoaffinity column for the simultaneous analysis of Biotin, Vitamin B12 and folic acid | EASI-EXTRACT® MULTI-VIT B (LGE)

- Single extraction method.

- Helps remove sample matrix interferences.

- Uses monoclonal antibodies.

- Extraction uses buffer and enzymes.

- Can be used with pigmented samples.

- Can be stored at a temperature of 21 – 25 °C.

- These columns are suitable for testing a wide range of commodities.

* Accessories the customer can buy additionally:

- Immunoaffinity Column Rack

- Immunoaffinity Column Accessory Pack

* Video

3. Specifications of the immunoaffinity column for the simultaneous analysis of Biotin, Vitamin B12 and folic acid | EASI-EXTRACT® MULTI-VIT B (LGE)