FastGene qFYR Real-Time PCR System

Overview of the FastGene real-time PCR instrument

1. Instrument information

FastGene® qFYR is a highly precise instrument for quantitative polymerase chain reaction (qPCR) experiments. qPCR is an established, efficient, and highly sensitive method for detecting and quantifying nucleic acids. During measurement, the target DNA or RNA sequence is amplified, and the cycle-dependent increase in fluorescence is detected in real time.

An instrument for many applications
FastGene® qFYR was developed to meet the highest laboratory standards and to deliver reliable performance for different real-time qPCR applications:

• Gene expression analysis
• Absolute and relative quantification
• Endpoint qualitative analysis
• Genotyping
• Gene mutation analysis
• Pathogen detection
• GMO detection
• Protein stability screening
• miRNA analysis
• Melt-curve analysis

2. Technical specifications:

Advanced technology of the FastGene real-time PCR instrument

1. High-sensitivity optical design

The FastGene® qFYR optical detection system combines a high-quality PMT (photomultiplier tube) with a short-focal-length Fresnel lens. The short distance from the detector to the sample reduces signal loss and cross-talk between samples and improves signal sensitivity overall.

2. Outstanding thermal-cycling performance

FastGene® qFYR delivers excellent temperature control, temperature accuracy (± 0,2 °C), and uniformity across the whole 96-well plate. The unique hollow thermal-block design reduces the overall weight of the FastGene real-time PCR instrument and ensures fast heating and cooling (up to 6 °C per second) for rapid qPCR protocols. The system also uses the most advanced Peltier components for the highest reaction quality and performance stability, giving reliable, consistent qPCR results.

Four high-performance colour LED channels

Because the distance between the scan head and the plate is small, the instrument FastGene® qFYR has outstanding sensitivity and almost no cross-talk between individual wells. Four different colour channels are installed in the scan head so that all commonly used qPCR dyes can be excited in a single plate scan (8 seconds). The dual FAM/SYBR channel allows melt-curve and high-resolution experiments in fast dual mode, cutting the measurement time in this channel by half.

• 4 different fluorescence channels and one dual FAM/SYBR channel allow a fast measurement.
• Detection channels:

Channel 1: FAM/SYBR Green, etc.
Channel 2: VIC/JOE/HEX/TET, etc.
Channel 3: ROX/Texas Red, etc.
Channel 4: Cy5, etc.
Channel 5: FAM/SYBR Green, etc.

Advantages of the FastGene instrument:

High-quality data
Outstanding uniformity
1.3-fold target discrimination
Accurate quantification
Robust multiplexing
High-resolution melt-curve analysis
qPCR gradient for optimal annealing
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