Compact Dry EC Ecoli Coliform plate | Compact Dry EC Ecoli Coliform | Shimadzu

– The Compact Dry Ecoli Coliform medium plate is a plate with a pre-poured freeze-dried medium, manufactured by the well-known pharmaceutical company Nissui Pharmaceutical.
– The Compact Dry Ecoli Coliform plate is a medium plate that can test both indicators, Ecoli and Coliform.
– To learn more about this convenient product, we will look at the advantages and the procedure of this convenient medium plate.

1. Overview of the Ecoli Coliform bacteria

– Escherichia coli and coliforms are found in the intestines of warm-blooded animals.
– Not all E. coli are pathogenic, but some strains can be regarded as opportunistic bacteria that infect a host with a weakened immune system.
– Testing for the presence of Coliform and E. coli is important and necessary in the food industry, especially in water quality monitoring.
– For this reason, a test procedure for Ecoli Coliform is recommended; and the Compact Dry Ecoli Coliform plate offers the user convenience thanks to the advantage of needing no preparation of culture-medium chemicals, equipment or complex consumables.
– All the technology used for this Compact Dry Ecoli Coliform plate is a pre-poured freeze-dried agar medium. The user only needs to extract the sample, pipette the sample onto the plate and take it for incubation.

2. Advantages of using the Compact Dry Ecoli Coliform plate

– Simple, easy-to-use procedure, with no need to prepare consumables or complex equipment.
– Easy to use and no time spent training users.
– The compact dry plate is easy to store thanks to its packaging of 4 plates per pack.
– The results are quite accurate, easy to analyse and available after 24 hours.
– Colonies can be picked for biochemical testing.

3. Test procedure on the Compact Dry Ecoli Coliform plate

* Preparing the Compact Dry plate

– Open the aluminium pouch.
– Take out a set of 4 plates, or take the 1 – 3 plates you need and keep the pouch sealed to avoid loss of quality due to light and humidity.
– Write the relevant information in the notes section.

* Sample preparation

– Prepare the diluent (Butterfield’s phosphate-buffered diluents): Weigh 34 g of KH2PO4 into 500 ml of distilled water. Adjust the pH to 7.2 with 1N NaOH. Sterilise for 15 minutes at 121° C.
– Prepare the sample dilution:
– Analyse 50 g of each sample. Add 450 ml of diluent. Homogenise with a sample blender for 2 minutes to obtain a 1:10 dilution.
– Take 1 ml of the 1:10 dilution and add it to 9 ml of diluent. Mix well to obtain a 1:100 dilution.

* Inoculating the sample

– Use a pipette to take 1 ml of sample.
– Open the lid of the plate.
– Inoculate 1 ml of sample in the centre of the plate.
– Close the lid of the plate and take it for incubation.

* Reading the results and counting colonies

- Choose plates with 10-150 colonies.

- All colonies that are blue or blue-violet are E.Coli.

- Colonies that are red or pink are Coliforms.

- All colonies that are red, pink, blue or blue-violet are total Coliforms.

* The handling procedure is illustrated in pictures as follows:

* Note

- A plate with more than 250 CFU may cause the whole colony area to turn red/pink. In this case the sample needs to be diluted.

- After the results are read, the plate is autoclaved (121 °C/ 30 minutes) before disposal.