Glucose, also known as grape sugar, is an important monosaccharide that serves as the primary energy source for the living a
1. Which foods contain glucose
Glucose occurs naturally in many foods, especially these groups:
- Fruit: grapes, bananas, apples, pears, mangoes…
- Vegetables: carrots, sweet potatoes, maize…
- Honey is one of the richest natural glucose sources.
- Processed products: confectionery, soft drinks, jams, condensed milk… often add glucose syrup for sweetness and body
- Cereals, wheat flour: during starch breakdown, glucose is released and absorbed into the blood.
Controlling the glucose content of food is essential — not only for taste and product quality, but also to meet
2. Analyzing glucose in food by the enzymatic method
To determine the glucose content of food precisely, the enzymatic method is regarded as the gold standard thanks to its high specificity, reliable results and broad matrix compatibility.
* The principle of the enzymatic method
The enzymatic reaction uses specific enzymes such as glucose oxidase (GOD) and peroxidase (POD) to convert glucose into gluconic acid and hydrogen peroxide. The hydrogen peroxide then reacts with a chromogenic indicator in the pre
🧪 Kit overview
- Product name: Enzytec™ Liquid D-Glucose
- Product code: E8140
- Manufacturer: R-Biopharm AG (Germany)
- Kit format: ready-to-use liquid reagents
- Method: Enzymatic – UV (kinetic method)
- Requirement: a UV/VIS spectrophotometer (photometer or automatic analyzer)
- Detection limit: 4.0 mg/L
- Reaction time: 10 minutes
- Measurement wavelength: 340 nm
📦 Composition of the E8140 kit
The Enzytec™ Liquid E8140 kit includes the main reagents, pre-mixed for laboratory convenience:
🔬 The enzymatic reaction principle
The process consists of two successive enzyme reactions, as follows:
1. Phosphorylation of glucose:
2. Oxidation of G6P:
- The NADPH generated is measured at the 340 nm wavelength with a photometer, and the absorbance intensity is proportional to the glucose concentration in the sample.
✅ Key advantages of the Enzytec™ Liquid D-Glucose E8140
- Liquid format: no complicated reconstitution → saves time and reduces errors.
- Highly specific for glucose, unaffected by other sugars such as fructose and sucrose...
- Suitable for many sample types: fruit juice, milk, beer, soft drinks, processed foods, cereals, honey-based products, pro
- Certified: AOAC-verified and ISO-recognized for food and feed testing purposes.
- Compatible with laboratory automation (biochemistry analyzers).
🧴 Using the sample
- Food samples must be prepared correctly (centrifuged, filtered, diluted) before analysis.
- Samples should not be deeply colored or contain sediment, to avoid affecting the photometric measurement at 340 nm.
📄 Certifications & standards
- Complies with the standards:
+ AOAC Official Method
+ EN ISO 27971 / 22514 (glucose analysis in cereals, fermented foods)
+ ISO 17025-ready if implemented in an accredited testing laboratory
📞 Contact us for a consultation and to order
Pacific Company – the official distributor of R-Biopharm Enzytec™ test kits in Vietnam. We provide:
- Free technical advice
- Setup and usage guidance on popular instruments