1. What is the enzymatic method?
- What is the enzymatic method? It is an analytical technique based on the specific catalytic activity of enzymes to determine the concentration of compounds such as sugars (glucose, fructose, sucrose, lactose…) or acids (lactic acid, citric acid, acetic acid…).
- The enzyme reacts with the analyte to produce a product that can be measured with a spectrophotometer, usually at a wavelength of 340 nm via the NAD(P)H/NAD(P)+ reaction.
- The high specificity of enzymes gives this method accurate results, high sensitivity, and almost no interference from other components in the sample.
2. Why use the enzymatic method to determine sugars and acids?
- High accuracy and specificity: enzymes react with only one specific substrate, so the enzymatic method can clearly distinguish each type of sugar or acid, helping to accurately analyse each individual component in a complex mixture.
- Suitable for many types of food: from juices, milk, beer and wine to fermented products, the enzymatic method can be applied effectively without a complex sample preparation process.
- Fast and convenient: with ready-made enzymatic test kits, users only need to follow simple instructions without investing in expensive analytical equipment or highly qualified technicians.
- Meets international standards: results obtained by the enzymatic method are generally accepted in the food testing standards of AOAC, ISO and international validation bodies.
3. R-Biopharm – A comprehensive enzymatic test kit solution
- R-Biopharm (Germany) is one of the world's leading suppliers of high-quality enzymatic test kits to determine the sugar and acid content in food. R-Biopharm's kits are designed on the enzymatic principle and have proven their effectiveness and reliability in testing laboratories worldwide.
* R-Biopharm's notable products include:
- Test kits for determining glucose, fructose, sucrose, lactose, maltose...
- Test kits for quantifying citric acid, lactic acid, acetic acid, malic acid…
- Optimised for spectrophotometers and easy to integrate into existing testing workflows.
With support from R-Biopharm, laboratories and food manufacturing businesses can be fully confident in the accuracy, efficiency and compliance with international food safety regulations.
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Determining sugar content in food by the enzymatic method
Determining acid content in food by the enzymatic method