- In the field of food and feed safety testing, the analysis of the mycotoxin Aflatoxin is mandatory to ensure consumer health and comply with strict toxin limit regulations.
- To achieve accurate and reliable analytical results, the use of certified Aflatoxin reference standards is essential. Among them, the Aflatoxin reference standard lines from Trilogy (USA) have been and are the preferred choice of many laboratories worldwide.
- Trilogy Analytical Laboratory (USA) is a leading global supplier of high-quality, certified and fully traceable mycotoxin reference standards. The Aflatoxin reference standard group includes:
1. Overview of Aflatoxin and the need for analysis
Aflatoxin is a group of mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus. The main toxins include:
- Aflatoxin B1 (most toxic)
- Aflatoxin B2
- Aflatoxin G1
- Aflatoxin G2
- They are commonly found in agricultural products such as corn, peanuts, rice, wheat, coffee, dried spices... with the risk of liver cancer and immune system damage.
- International standards such as Codex, EU, US FDA and ASEAN all set very strict limits on Aflatoxin levels in food, especially the limits for Aflatoxin B1 and total Aflatoxin (Aflatoxin Total).
2. Key features of the Trilogy Aflatoxin reference standards
🔹 Trilogy® Liquid Standard Aflatoxins B1, B2, G1, G2
- Trade name: Liquid Standard Aflatoxins B1, B2, G1, G2
- Product code: TSL-108-10
- Pack size: 10 ml/vial
- Form: aflatoxin B1, B2, G1, G2 (4:1:4:1) 5 μg/mL (2/0.5/2/0.5 μg/mL) in acetonitrile solvent
- Composition: Aflatoxin B1, B2, G1, G2 mixture at accurate concentrations
- Use: instrument calibration and building calibration curves in total Aflatoxin analysis.
🔹 Trilogy® Liquid Standard Aflatoxins B1
- Trade name: Liquid Standard Aflatoxins B1
- Product code: TSL-104-10
- Pack size: 10 ml/vial
- Form: aflatoxin B1 25 μg/mL in acetonitrile solvent
- Composition: Aflatoxin B1 mixture at accurate concentration
- Use: instrument calibration and building calibration curves in Aflatoxin B1 analysis.
🔹 Trilogy® Liquid Standard Aflatoxins B2
- Trade name: Liquid Standard Aflatoxins B2
- Product code: TSL-105-10
- Pack size: 10 ml/vial
- Form: aflatoxin B2 25 μg/mL in acetonitrile solvent
- Composition: Aflatoxin B2 mixture at accurate concentration
- Use: instrument calibration and building calibration curves in Aflatoxin B2 analysis.
🔹 Trilogy® Liquid Standard Aflatoxins G1
- Trade name: Liquid Standard Aflatoxins G1
- Product code: TSL-106-10
- Pack size: 10 ml/vial
- Form: aflatoxin G1 25 μg/mL in acetonitrile solvent
- Composition: Aflatoxin G1 mixture at accurate concentration
- Use: instrument calibration and building calibration curves in Aflatoxin G1 analysis.
🔹 Trilogy® Liquid Standard Aflatoxins G2
- Trade name: Liquid Standard Aflatoxins G2
- Product code: TSL-107-10
- Pack size: 10 ml/vial
- Form: aflatoxin G2 25 μg/mL in acetonitrile solvent
- Composition: Aflatoxin G2 mixture at accurate concentration
- Use: instrument calibration and building calibration curves in Aflatoxin G2 analysis.
🔹 Trilogy® Liquid Standard Aflatoxins M1
- Trade name: Liquid Standard Aflatoxins M1
- Product code: TSL-143-2
- Pack size: 2 ml/vial
- Form: aflatoxin M1 0.5 μg/mL in acetonitrile solvent
- Composition: Aflatoxin M1 mixture at accurate concentration
- Use: instrument calibration and building calibration curves in Aflatoxin M1 analysis.
3. Applications of Aflatoxin reference standards in testing
Trilogy Aflatoxin reference standards are widely used in the following analytical methods:
- High-performance liquid chromatography HPLC, HPLC-MS/MS.
- Gas chromatography GC-MS.
- ELISA or absorption spectrophotometry.
* Practical applications
- Testing raw materials (cereals, oilseeds).
- Used as reference standards for building calibration curves in HPLC and LC-MS/MS analysis.
- Evaluating recovery, limit of detection (LOD) and limit of quantification (LOQ) in analytical methods.
- Inter-laboratory comparison, developing or qualifying analytical procedures.
- Monitoring processed products (milk, feed, dried food).
- Import/export inspection of agricultural products.
- Research and method validation.
4. Advantages of Trilogy Aflatoxin reference standards
- High purity, stable and fully certified.
- Compliant with ISO 17034 reference material standards.
- Complete COA and MSDS.
- Compatible with many modern analytical methods and instruments.
- Convenient: ready-to-use solution form, saving preparation time.
5. How to use
* Step 1: Prepare the calibration curve
- Take a suitable amount of reference standard (e.g. 50 µL, depending on concentration) from the stock vial.
- Dilute in solvent (usually methanol or a methanol : water mixture) to prepare the required standard levels.
- The concentration levels are prepared to build a linear calibration curve for quantification.
* Step 2: Inject the sample into the analytical instrument
- HPLC system with fluorescence detector (HPLC-FLD, usually post-column derivatization with bromine).
- Set chromatographic conditions appropriate for each aflatoxin.
* Step 3: Process and compare the data
- Compare the peak areas of the test sample with the standard levels to calculate the aflatoxin content in the sample.
6. Aflatoxin reference standard product catalog information
Aflatoxin reference standards from Trilogy® are the trusted choice for food and feed laboratories and testing organizations seeking high accuracy in mycotoxin analysis. Using internationally certified reference standards not only ensures the quality of the analysis but also helps companies meet quality control requirements and food safety legislation.
If you are looking for an optimal solution for Aflatoxin analysis, choose Trilogy – reliable Aflatoxin reference standards for modern laboratories.