- However, sulfite residues exceeding the limit can affect consumer health, especially for people who are sensitive or have asthma. Therefore, the use of rapid sulfite testing plays an essential role in ensuring safety and compliance with international regulations.
- One of the advanced and reliable solutions available today is the kit Enzytec™ Generic Sulfite (SO₂-Total), item no. E6275, developed using the Enzymatic method.
1. Enzytec™ Generic Sulfite (SO₂-Total) – E6275 product information
- Product name: Enzytec™ Generic Sulfite (SO₂-Total)
- Item no.: E6275
- Principle: Sulfite in the sample reacts with the enzyme in the kit, producing a change in absorbance, from which the total SO₂ content is quantified accurately.
- Measurement type: analysis by UV/VIS spectrophotometer.
- Run time: fast, results within 20–30 minutes.
- Kit format: ready-to-use format, suitable for many laboratories.
2. Operating principle
The Enzytec™ E6275 kit works on the basis of enzymatic – photometric reaction (enzyme-based photometric assay). Specifically:
2.1. Release of free and bound SO₂
Food/beverage samples containing sulfite (in free or bound SO₂ form) are chemically treated in the kit to release all sulfite components into the SO₂-Total.
2.2. Specific enzymatic reaction
- Sulfite after release reacts with the enzyme sulfite oxidase (SOX), converting into sulfate (SO₄²⁻).
- During this oxidation, hydrogen peroxide (H₂O₂) is also produced.
2.3. Color reaction (colorimetric detection)
- The H₂O₂ produced is catalyzed by peroxidase enzyme to react with a color indicator contained in the kit.
- The reaction forms a colored compound whose intensity is proportional to the initial sulfite concentration in the sample.
2.4. Photometric measurement and quantification
- The colored compound is measured at a specified wavelength on a UV/VIS spectrophotometer.
- The absorbance value is compared with a calibration curve, from which the total SO₂ content (SO₂-Total) in the food or beverage sample is calculated.
- Thanks to the mechanism based on specific enzyme reactions and accurate spectrophotometric measurement, the Enzytec™ Generic Sulfite kit delivers reliable results, eliminating interference from complex food matrices.
3. Highlights of the Enzytec™ E6275 rapid sulfite test kit
- High accuracy: the Enzymatic method eliminates interference from complex matrix components, giving reliable results.
- Fast and convenient: short analysis time, supporting rapid testing in the lab.
- Wide application: suitable for many different types of food and beverages.
- Compliance with international standards: recognized in professional food testing laboratories.
- Easy to operate: simple procedure, suitable even for newly trained technicians.
4. Applications of the rapid sulfite test
- Checking SO₂ levels in wine, beer, and alcoholic beverages.
- Quantifying sulfite in fruit juice, soft drinks.
- Detecting sulfite in seafood, dried vegetables and fruits, jam, and processed foods.
- Used in testing laboratories, food manufacturing plants, research institutes, and food safety authorities.
5. Rapid sulfite test procedure
- Sample preparation: take the food or beverage sample to be tested and pre-treat it according to the kit instructions.
- Performing the reaction: add Enzytec™ reagent to the sample according to the preset steps.
- Enzymatic reaction: sulfite in the sample reacts with the enzyme, producing a measurable product.
- Spectrophotometry: place the cuvette into the UV/VIS spectrophotometer to measure the absorbance at the specified wavelength.
- Calculating results: compare the absorbance value with the standard to determine the total SO₂ content in the sample.
Sulfite is an important parameter to monitor in the food and beverage industry. With the kit Enzytec™ Generic Sulfite (SO₂-Total), item E6275, manufacturers and testing laboratories can easily deploy rapid sulfite testing with high accuracy, a simple procedure, and a short run time. This is an optimal solution to ensure product quality, protect consumer health, and meet international standards.
👉 Refer to the product Enzytec™ Generic Sulfite (SO₂-Total), item E6275
