Salmonella, Listeria and the bacterium E. coli that produce Shiga toxin (STEC) are the cause of thousands of foodborne illness outbreaks worldwide every year. This leads to serious health consequences, product recalls and significant economic damage. Rapid and accurate detection of these pathogens is very important to maintain food safety, prevent disease outbreaks and ensure regulatory compliance with the Real-time PCR kits (qPCR) for Salmonella and Listeria testingfrom R-biopharm, based on qPCR. The kits are designed to detect quickly, reliably and accurately these pathogens, helping to protect community health and to safeguard your business.
SureFast® ONE – Real-time PCR kits for foodborne pathogen detection — AOAC / MicroVal certified
Why choose the Real-time PCR kit SureFast® ONE qPCR Kits?
- Easy to use: the thermal lysis step is completed in 10 minutes, with simple handling.
- Fast: all Real-time PCR kits in the ONE line can be run at the same time.
- Multi-instrument compatibility: works with common cyclers (FAM, HEX/VIC, ROX, Cy5).
- AOAC / MicroVal certification: ensures independent accuracy and reliability.
- 24-month shelf life: reduces waste and saves costs.
- In-depth support: provides complete guidance and training.
- Scalability: suitable for small to large laboratories
The main products in the Real-time PCR kit SureFast® ONE range:
- SureFast® Salmonella ONE: Real-time PCR kit for detecting Salmonella spp. Evaluated according to ISO 16140‑2 and certified by MicroVal (2014LR43) and AOAC PTM (081803).
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- SureFast® Listeria 3plex ONE: Real-time PCR kit for detecting Listeria spp. and L. monocytogenes at the same time. It is AOAC PTM certified (062501) and comes with the DNA standard preparation integrated in the kit for immediate use after the 10-minute lysis step.
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- SureFast® STEC 4plex ONE: Real-time PCR kit for detecting the toxin genes stx1 (a-d), stx2 (a-g), eae and E. coli O157.
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See also: Catalogue of SureFast microbiology products
Simple qPCR workflow:
- Culture the food sample (usually after the enrichment step).
- Perform the thermal lysis step for 10 minutes to break open the cells – no further DNA extraction is needed.
- Mix the DNA directly into the qPCR reaction mix to amplify and detect quickly.
The complete SureFast® ONE & RIDA®CYCLER
qPCR analysis made easier than ever
Compact: The portable 4-channel qPCR device weighs only 2,1 kg and fits in the palm of your hand.
Modular and flexible design: several devices can be connected to one computer wirelessly via Bluetooth.
Easy to use: the intuitive software with pre-installed thermal profiles ensures simple operation without programming and starts the analysis quickly.
Efficient: several different PCR tests can be processed at the same time with the same thermal profile.
Powerful: The patented induction technology and the integrated fan ensure fast heating and cooling with excellent temperature uniformity. With a fixed optical path and no moving parts, the device needs no calibration.
https://pacificlab.vn/vi/shops/phong-thi-nghiem/thiet-bi-qpcr-r-biopharm.html
Product introduction video
If you would like to learn more about Real-time PCR kits for Salmonella and Listeria testing, please contact us by leaving your details via the contact form or by contacting the PacificLAB team:
PACIFICLAB SCIENTIFIC AND TECHNICAL EQUIPMENT CO., LTD
 | Telephone (+84) 28 62 65 46 83 Hotline: 0989 092 258 |  | Fax (+84) 28 62 65 46 93 |
 | Email sales@pacificlab.vn |  | Website www.pacificlab.vn |
