Immunoaffinity columns | An effective solution for mycotoxin analysis

In food safety testing, mycotoxins are a worrying biological hazard, directly affecting the health of humans and livestock. To analyze these toxins accurately, immunoaffinity column technology has become an indispensable tool in modern laboratories. In particular, R-Biopharm (Germany) immunoaffinity columns are highly rated for their exceptional accuracy, cleanliness, and repeatability.

26 April 20252 min read

1. What is an immunoaffinity column

- An immunoaffinity column (Immunoaffinity Column — IAC) is a product used in chromatography, applying immunological principles to selectively capture and enrich target molecules — in this case mycotoxins such as Aflatoxin, Ochratoxin A, Fumonisin, Zearalenone, Deoxynivalenol (DON), and T-2/HT-2...

- The column is fitted with specific antibodies that can selectively "capture" the toxin to be analyzed from food or feed samples. After the impurities are washed away, the toxin is released from the column with a suitable solvent to be run through analytical techniques such as HPLC and LC-MS/MS.

2. Outstanding advantages of R-Biopharm immunoaffinity columns

- High selectivity and cleanliness: specific monoclonal antibodies remove impurities completely, improving the accuracy of the analysis results.

- Saves time and cost: minimizes complex sample preparation steps, suiting high-volume testing workflows.

- Versatile application: can be used for many sample types such as cereals, oilseeds, animal feed, coffee, cocoa, dried fruit, milk, beer...

- Optimized for many analytical techniques: fully compatible with HPLC, UHPLC, and LC-MS/MS — precise quantitative methods.

- Meets international standards: the products are certified by AOAC and CEN, and comply with EU and FDA regulations.

- Reliability from a leading brand: R-Biopharm is a leading manufacturer in food testing, renowned for the stability and quality of its products.

3. The mycotoxins that can be analyzed with R-Biopharm IAC columns

- Aflatoxin B1, B2, G1, G2

- Aflatoxin M1

- Ochratoxin A

- Zearalenone

- Fumonisin B1, B2

- Deoxynivalenol (DON)

- T-2 and HT-2 toxin

- Multi-toxin (several toxins analyzed simultaneously)

- R-Biopharm supplies single-toxin and multi-toxin column lines, giving flexibility in choice depending on the goal and scale of the analysis.

4. Applications of immunoaffinity columns in mycotoxin analysis

- Food raw material testing: cereals, oilseeds, spices, tea, coffee, cocoa...

- Animal feed and ingredient analysis: ensuring the safety of livestock and preventing illnesses associated with mycotoxins.

- Food quality surveillance: strict compliance with international standards.

- Research and development: in R&D projects, evaluating preservation effectiveness and antifungal performance...

The presence of mycotoxins in food is a major risk to public health. Applying immunoaffinity columns from R-Biopharm not only makes analysis fast and accurate but also raises product quality and reputation. It is the optimal choice for testing laboratories, food processors, and quality surveillance organizations.