One of the reliable ELISA product lines available today is RIDASCREEN® from R-Biopharm – a brand from Germany with more than 30 years of experience in food analysis. To perform the technique correctly and ensure accurate results, you need to fully equip the devices in both 2 stages: sample preparation and ELISA analysis.

1. Equipment needed for the mycotoxin ELISA test

1.1. Microplate reader

- Read optical density (OD) at the appropriate wavelength (usually 450 nm).

- Able to read 96-well plates.

1.2. Analytical balance

- Minimum accuracy of 0.001 g.

- Used to weigh test samples (cereals, food, animal feed…) with the exact mass according to the kit instructions.

1.3. Sample grinder / Laboratory mill

- Used to grind food or raw material samples down to a uniform size (fineness suitable for mycotoxin extraction).

- Can be a dry grinder, a blade-type grinder, or a dedicated laboratory mill.

1.4. Microplate shaker

- Used to mix solutions evenly in the wells, helping reactions occur uniformly.

- Choose a model that can precisely adjust the shaking speed.

1.5. Centrifuge

- Used in the sample preparation process (separating the sediment).

- Needs a model that can use 15 mL or 50 mL centrifuge tubes.

1.6. Micropipette set and pipette tips

- Single-channel and/or multichannel pipettes (1 – 1000 µL depending on the step).

- Ensure accuracy and avoid cross-contamination.

1.7. Vortex mixer

- Mix samples quickly, ensuring the solution is homogeneous.

- Helps mix samples uniformly with the extraction solvent.

1.8. Vacuum pump (if using an automatic washing system)

- Helps wash plates faster and more efficiently than manual washing.

- Useful when processing many samples, saving time.

2. Consumables needed for the mycotoxin ELISA test

- Sterile pipette tips

- Test tubes or centrifuge tubes (15 mL / 50 mL)

- Absorbent paper or lint-free wipes

- Filtration tools (syringe filter or filter paper if sample filtration is needed)

- Sample extraction solvent: methanol, acetonitrile or water depending on the specific ELISA test type.

- Distilled/deionized water: used to prepare wash solutions and other reagents.

- ELISA plates (usually already included in the RIDASCREEN® kit)

3. Notes when performing the R-Biopharm RIDASCREEN® mycotoxin ELISA test

- Each mycotoxin ELISA test RIDASCREEN® kit comes with specific instructions for each toxin type. Operators must strictly follow the procedure to ensure accurate results.

- The ELISA reader must be regularly calibrated and maintained to ensure accuracy when reading results.

- Use laboratory-grade consumables and always work in clean conditions.

Properly and correctly equipping equipment and consumables will help the mycotoxin ELISA test RIDASCREEN® from R-Biopharm proceed smoothly and yield reliable results. This is an optimal solution for food testing laboratories, especially in the field of quality control of agricultural raw materials, animal feed, and export products.

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