1. Overview of GMO testing by PCR

- Controlling and detecting GMOs is not only a food safety requirement, but is also directly related to legal regulations, product labeling and international exports.

- Among the analytical methods available today, PCR (Polymerase Chain Reaction) – especially real-time PCR – is regarded as the gold standard in GMO testing because it can:

+ Directly detect genetically modified DNA

+ Highly sensitive and specific

+ Applicable to both raw materials and processed foods

- R-Biopharm (Germany) is one of the world's leading manufacturers in the field of GMO PCR test kits, offering a complete range of solutions from general screening to event-specific GMO identification.

2. Common GMO screening targets by PCR

2.1. What is GMO screening?

- GMO screening is the initial filtering step, aimed at determining whether the test sample contains genetically modified DNA, before carrying out more in-depth analyses.

- Screening targets usually focus on gene sequences that are common and widely used in GMO breeding.

- R-Biopharm provides GMO screening PCR test kits designed to be optimal and suitable for many different matrices.

2.2. CaMV 35S Promoter – the most common GMO screening target

-The CaMV 35S promoter is a gene promoter sequence originating from the Cauliflower Mosaic Virus, very commonly used in GM crop varieties.

* Key features

- Present in most commercial GMOs today

- A screening target required in many international standards

- Suitable for quickly checking for the presence of GMOs

* Advantages of using the R-Biopharm CaMV 35S PCR test

- Highly sensitive, detecting DNA at very low levels

- Specific primer and probe design

- Stable, easy to use for testing laboratories

👉 This is a mandatory GMO screening target in many quality control programs.

2.3. NOS Terminator – another important screening target

- The NOS terminator is a transcription termination sequence originating from Agrobacterium tumefaciens, also widely used in genetic modification technology.

* The role of the NOS terminator in GMO testing

- Combined with CaMV 35S, it increases screening reliability

- Reduces the risk of false negatives

- Supports the detection of GMOs that do not carry the 35S promoter

* The R-Biopharm NOS terminator PCR test is optimized for

- Raw materials

- Feed

- Processed foods

3. Event-specific GMO targets

3.1. What is an event-specific GMO?

Unlike screening, event-specific PCR allows:

- Exact identification of a specific GM variety

- Distinguishing between different genetic modification events

- Serving the purposes of labeling, traceability and legal compliance

R-Biopharm is one of the few manufacturers offering a full range of GMO event-specific PCR kits, widely recognized around the world.

3.2. Soybean GMO – Soy GTS 40-3-2 (Roundup Ready)

- GTS 40-3-2 is the most common GM soybean variety in the world, resistant to the herbicide glyphosate.

* Testing applications

- Imported soybean raw materials

- Soybean products (soy flour, soy protein, lecithin)

- Feed

* The R-Biopharm Soy GTS 40-3-2 PCR test

- Accurately detects the genetic modification event

- Suitable for qualitative and quantitative analysis

- Widely used in export testing

3.3. Maize GMO – common events

- Corn is the GM crop with the largest number of genetic modification events today. R-Biopharm offers PCR test kits for many important events such as:

🔹 MON810

- Insect-resistant corn

- Widely grown in America and Europe

🔹 Bt11

- Insect-resistant, herbicide-tolerant corn

- Commonly found in animal feed

🔹 NK603

- Glyphosate-tolerant corn

- Common in raw materials for food and feed production

* Advantages of the R-Biopharm maize GMO PCR test

- High specificity for each event

- Reduces the risk of confusion between GMOs

- Suitable for international testing standards

4. Detecting multiple GMOs in one sample by PCR

In practice, a test sample may contain several different GMOs, especially:

- Feed

- Blended raw materials

- Processed foods

* The R-Biopharm PCR test system allows

- Rapid screening of multiple GMOs in a single workflow

- Flexible combination of screening and event-specific tests

- Optimizing analysis time and cost

This is especially important for service laboratories and large-scale manufacturers.

5. Flexibility and key advantages of the R-Biopharm GMO PCR test system

- Suitable for many types of matrix: raw materials, processed foods, feed.

- Easy to integrate into the laboratory system: compatible with many real-time PCR instruments, clear procedures, complete technical documentation, easy training.

- Internationally recognized: compliant with ISO, EN, AOAC, used in many public and private testing laboratories.

6. Conclusion

- GMO testing by the PCR method is an accurate, reliable solution that matches modern food safety management trends. With a diverse range of screening and event-specific targets, the GMO PCR test system from R-Biopharm fully meets the needs from rapid screening to detailed identification of each genetic modification event.

- For food and feed companies and testing laboratories, choosing the R-Biopharm GMO PCR test kit is an important step aimed at:

+ Complying with legal regulations.

+ Controlling quality risks.

+ Enhancing product reputation in domestic and international markets.

- A reputable provider of molecular biology testing solutions in Vietnam, Pacific company is proud to be a distribution partner of genuine R-Biopharm (Germany) GMO PCR test kits, fully meeting the testing needs of laboratories, food companies and feed factories.

- We not only supply high-quality products, but also accompany our customers through:

+ Advice on choosing GMO targets suitable for each type of sample and testing purpose

+ Technical support and PCR operation training for laboratories

+ Synchronized solutions from GMO screening to event-specific, to international standards

+ Stable supply, clear origin, complete technical documentation and certificates

- With many years of experience in microbiology – molecular biology – food safety, Pacific company is committed to providing GMO PCR solutions.

📞 Contact Pacific company today for detailed advice on:

- R-Biopharm GMO PCR test kits

- The range of screening & event-specific targets

- GMO PCR implementation solutions for your laboratory

👉 Pacific company – accompanying quality and reliability in GMO testing